Journal: Annals of Translational Medicine

Article Title: Cornel iridoid glycoside ameliorates cognitive deficits in APP/PS1/tau triple transgenic mice by attenuating amyloid-beta, tau hyperphosphorylation and neurotrophic dysfunction

doi: 10.21037/atm.2020.02.138

Figure Lengend Snippet: The effects of CIG on activity-related modifications of PP2A and GSK-3β in the cerebral cortex and hippocampus of 3×Tg mice (western blotting). (A) Representative blots of PP2A catalytic subunit C (PP2Ac) methylated PP2Ac at Leu309 (m-PP2Ac), GSK-3β and phosphorylated GSK-3β at Ser9 (p-GSK3β-Ser9). Quantitative findings of PP2Ac, m-PP2Ac, GSK-3β and p-GSK3β-Ser9 in cerebral cortex (B) and hippocampus (C). Internal control was β-actin. Ratio of target protein to β-actin in WT control mice was set as 1. Data are expressed as means ± SEM; n=4 per group. ###, P<0.001, 3×Tg group vs. WT group; *, P<0.05, **, P<0.01, ***, P<0.001, CIG-treated groups vs. 3×Tg group. CIG, cornel iridoid glycoside.

Article Snippet: The monoclonal primary antibodies used in these assays comprised rabbit anti-Aβ antibody diluted 1:1,000; mouse anti-CREB antibody diluted 1:1,000 and rabbit anti-phosphorylated CREB antibody diluted 1:1,000 (Cell Signaling Technology), rabbit anti-APP antibody diluted 1:500, rabbit anti-BACE1 antibody diluted 1:10,000, rabbit anti-IDE antibody diluted 1:10,000, rabbit anti-p-GSK3β-Ser9 antibody diluted 1:1,000 and rabbit anti-NGF antibody diluted 1:1,000 (Abcam, Cambridge, UK); rabbit anti-ADAM10 antibody (diluted 1:10,000; Sigma), rabbit anti-NEP antibody diluted 1:1,000 (Merck-Millipore); rabbit anti-PP2Ac antibody diluted 1:500 and mouse anti-methylated PP2Ac antibody diluted 1:100 (Santa Cruz Biotechnology Inc., Dallas TX, USA); rabbit anti-GSK-3β antibody diluted 1:1,000 and mouse anti-β-actin antibody diluted 1:1,000 (ZSGB-Biotechnology Company, Beijing, China); rabbit anti-BDNF antibody diluted 1:2,000 (Epitomics International Inc., Burlingame, CA, USA); rabbit anti-phosphorylated tau at Ser396 antibody diluted 1:1,000 and rabbit anti-phosphorylation tau at Ser44 site antibody diluted 1:1,000 (Invitrogen, Waltham, MA, USA).

Techniques: Activity Assay, Western Blot, Methylation, Control

Journal: Neurobiology of aging

Article Title: Administration of the benzodiazepine midazolam increases tau phosphorylation in the mouse brain

doi: 10.1016/j.neurobiolaging.2018.10.027

Figure Lengend Snippet: List of antibodies used

Article Snippet: demethylated PP2A-C , , Monoclonal , Mouse , Cell Signaling , Danvers, MA , 4466 , 1:1000.

Techniques:

Journal: Journal of neuroscience research

Article Title: Hindbrain A2 Noradrenergic Neuron Adenosine 5′-Monophosphate-Activated Protein Kinase (AMPK) Activation, Upstream Kinase/Phosphorylase Protein Expression, and Receptivity to Hormone and Fuel Reporters of Short-Term Food Deprivation are Regulated by Estradiol

doi: 10.1002/jnr.23892

Figure Lengend Snippet: Description of Primary Antibodies Used

Article Snippet: PP2A-C alpha/beta (7A6) , Unmethylated C-terminus amino acids 302–309 of human PP2A-C , Santa Cruz Biotechnol.; mouse; sc-81601; Lot no. J1410; AB_1128773 , 0.4 ug/mL.

Techniques: Concentration Assay