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Image Search Results
Journal: Annals of Translational Medicine
Article Title: Cornel iridoid glycoside ameliorates cognitive deficits in APP/PS1/tau triple transgenic mice by attenuating amyloid-beta, tau hyperphosphorylation and neurotrophic dysfunction
doi: 10.21037/atm.2020.02.138
Figure Lengend Snippet: The effects of CIG on activity-related modifications of PP2A and GSK-3β in the cerebral cortex and hippocampus of 3×Tg mice (western blotting). (A) Representative blots of PP2A catalytic subunit C (PP2Ac) methylated PP2Ac at Leu309 (m-PP2Ac), GSK-3β and phosphorylated GSK-3β at Ser9 (p-GSK3β-Ser9). Quantitative findings of PP2Ac, m-PP2Ac, GSK-3β and p-GSK3β-Ser9 in cerebral cortex (B) and hippocampus (C). Internal control was β-actin. Ratio of target protein to β-actin in WT control mice was set as 1. Data are expressed as means ± SEM; n=4 per group. ###, P<0.001, 3×Tg group vs. WT group; *, P<0.05, **, P<0.01, ***, P<0.001, CIG-treated groups vs. 3×Tg group. CIG, cornel iridoid glycoside.
Article Snippet: The monoclonal primary antibodies used in these assays comprised rabbit anti-Aβ antibody diluted 1:1,000; mouse anti-CREB antibody diluted 1:1,000 and rabbit anti-phosphorylated CREB antibody diluted 1:1,000 (Cell Signaling Technology), rabbit anti-APP antibody diluted 1:500, rabbit anti-BACE1 antibody diluted 1:10,000, rabbit anti-IDE antibody diluted 1:10,000, rabbit anti-p-GSK3β-Ser9 antibody diluted 1:1,000 and rabbit anti-NGF antibody diluted 1:1,000 (Abcam, Cambridge, UK); rabbit anti-ADAM10 antibody (diluted 1:10,000; Sigma), rabbit anti-NEP antibody diluted 1:1,000 (Merck-Millipore); rabbit anti-PP2Ac antibody diluted 1:500 and
Techniques: Activity Assay, Western Blot, Methylation, Control